摘要
为了从遗传学基础上，探索抗Ｏ／１２９的非Ｏ１群霍乱弧菌的基因结构的特点，即对１９９２～１９９４年从肠道门诊急性腹泻患者粪便中分离到的２１株抗Ｏ／１２９的非Ｏ１群霍乱弧菌的分子生物学特性进行了研究。结果显示：染色体ＤＮＡ经核酸限制性内切酶ＰｓｔＩ消化后，用琼脂糖凝胶电泳分离ＤＮＡ，全部菌株表现为多样化特征的限制性酶谱；用Ｄｉｇ标记的基因探针做ｓｏｕｔｈｅｒｎｂｌｏｔ杂交，无一株菌具有Ｏ１群霍乱弧菌流行株所具有的ＣＴ毒素基因；质粒图谱分析，仅有６株菌各含有一条质粒带，而且这６株菌同时对３～８种临床常用抗生素耐药，表明该菌株对Ｏ／１２９的抗性是非质粒性的。结果表明，抗Ｏ／１２９的非Ｏ１群霍乱弧菌与Ｏ１群霍乱弧菌非流行株具相似的遗传学特性和毒力特征，因而，它不是引起霍乱暴发的病原体。
his paper reported theresults of molecular biological study on 2 1 strains of vibriostatic agent O/129-resistant vibrio cholerae non-O1 isolated from patients of acute diarrhoea admitted to Beijing TianTan Hospital from May 1992 to Octorber 1994.The results showed that the restriction patterns of chro-mosomal DNA of the strains were diverse after the chromosomal DNAs being digested with Pst 1 andseparated by agarose gel electrophores. By southern blot hybridization analysis with gene probe labelledwith Dig,non of the strains possessed the fragments of CT as the control strain of the vibro choleraeO1.The examination for the presence of plasmid DNA showed that plasmid bands were not detected inthe O/129-resistant strains except 6 strains which had the presence of one plasmid and were also foundto be resistant to 3-8 antibiotics commonly used in clinical practices. Thus O/129 resistance is not to beplasmid mediated in the strains tested.The results demonstrated that O/129-resistant vibrio choleraenon-O1 were similar to those non-epidemic strains of vibrio cholerae O1 on genotype and toxin genescharacteristic. It is concluded that O/129-resistant vibrio cholerae non-O1 were not to be the pathogenof vibrio cholerae outbreak.
基金
首都医科大学附属北京天坛医院科研基金
