摘要
采用聚合酶链式反应-单链构型多态分析,对17例原发性卵巢癌组织p53基因外显子4~8进行了检测。结果检出突变5例,等位基因丢失5例,各占29.4%。对1例突变测序分析发现单个碱基置换,导致提前产生1个终止密码。在临床Ⅱ~Ⅳ期癌组织中,均检出p53基因的改变。故认为p53基因改变可能发生在卵巢癌的早期,并持续存在于肿瘤发展的全过程,是卵巢癌发生发展的危险因素之一。
The exons 4-8 of the p53 gene were examined in 17 human primary ovarian cancers by using polymerase chain reaction-single strand conformation polymorphism analysis.The results showed 5 cases of mutations (29.4%)and 5 cases of allelic losses(29.4%), respectively. A single base substitution was found by DNA sequencing analysis in one of these mutational cases, which resulted in the emergence of a stop code ahead of time. The alterations of p53 gene commonly detected at the clinical stages Ⅱ-Ⅳ, suggesting that the alterations of the p53 gene are early events and they could go through the whole course of tumor development. The loss of normal function of p53 gene is one of the critical events of the development of ovarian cancer.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1995年第2期69-71,共3页
Chinese Journal of Medical Genetics
基金
国家自然科学基金
关键词
卵巢肿瘤
癌
P53
基因突变
序列
测定
Ovarian cancer p53 gene Single strand conformation polymorphism Sequence