冷冻治疗急性出血坏死性胰腺炎的实验研究

Cryosurgery of acute hemorrhagic necrotizing pancre-atitis

作者对通过自体胆汁胰管逆行注射法制作的犬急性出血坏死性胰腺炎模型进行了冷冻治疗研究。冷冻温度一１６０°～一１９０℃，冷冻时间１５～１８秒，受冻面积约占胰表面的８０％。结果显示：冷冻治疗后胰腺炎症明显抑制；冷冻后２４小时胰酶活性开始下降，７天内血清磷脂酶Ａ２（ＰＬＡ２）、脂肪酶和淀粉酶下降分别为冷冻升高值的５０２．１％、３５５．８％和５０２．５％，血糖相对降低；冷冻后第２周胰腺纤维组织增生包绕残存腺泡和胰岛细胞，细胞内酶原颗粒丰富，分泌颗粒完整，血糖达正常水平。实验组和对照组在４８小时内的病死率分别为６．８９％和１００％，说明冷冻治疗急性出血坏死性胰腺炎犬效果良好。

cute hemorrhagic necrotizing pancreatitis (AH-NP)was induced by introduct retrograde injection ofthe autologous bile in canine. The cryoprobe was un-der the influence of temperature ranging from -160Cto-190C for fifteen to eighteen seconds, and thefrozen areas were about 80% surface of pancreas.Cryotherapy of AHNP strongly inhibited the develop-ment of the inflammation in pancreatic parenchyma.Postcryotherapy 24hr for remarkably decreased the ac-tivities of pancreatic enzymes. The activities of phos-pholipase A , lipase and amylase in serum decreased by502.1% , 355. 8%, 502. 5% respectively in 7 days.The level of blood glucose decreased. At the secondweek , the fibroblasts were growing around the residualpancreatic aciner cells and insular cells rich in intact zy-mogen granules. The mortility (6. 89%) of the treat-ment group within 48hrours was significantly lowerthan that of the control group (100%) .