白细胞介素6基因疗法对小鼠造血系统的调控作用

Experimental study on in vivo hematopoietic regulationof IL-6 gene therapy

通过研究成纤维细胞介导的人白细胞介素６（ＩＬ－６）基因疗法对小鼠造血系统的影响，发现：经ＩＬ－６基因转移的高分泌ＩＬ－６的成纤维细胞株移植于小白鼠体内后，第４天血小板数量开始上升，于第１０天达高峰，并持续到第２２天；中性粒细胞也上升，但白细胞总数变化不明显；骨髓和脾脏的粒细胞／巨噬细胞克隆形成单位（ＣＦＵ－ＧＭ）及巨核细胞克隆形成单位（ＣＦＵ－ＭＫ）数量均有不同程度的上升。表明成纤维细胞介导的人ＩＬ－６基因疗法能显著增强机体骨髓和脾脏造血功能，提高外周血血小板和中性粒细胞的数量，为临床上用该疗法治疗血小板减少症及肿瘤患者放化疗后机体造血系统重建提供了实验依据。

nterleukin -6 (IL-6) , a pleiotropic cytokine, isinvolved in extensive immune regulation and hematopoi-etic regulation. We observed the effect of fibroblast me-diated human IL-6 gene therapy on hematopoiesis.The platelete counts started to increase at day 4 afterimplantation of IL- 6 highly secreting fibroblast cellsand peaked at day 10 and lasted at high level for 22davs. The neutrcohil counts were elevated after theirimplantation, but WBC did not show any remarkableincrease. The CFU-GM and CFU-MK in bone marrowand spleen were also increased significantly. The resultsdemonstrated that fibroblasts mediated human IL-6gene therapy can significantly augment in vivohematopoietic functions in bone marrow and spleen andelevate the number of nutrophils and plateletes. Thisstudy provides a new approach to treat thrombopeniaand chemotherapy or radiotherapy induced hematopoi-etic suppression.