大鼠肠系膜微淋巴管自律运动特征的研究

The characteristics of spontaneous rhythmical mi-crolymphomotion

利用活体微循环显微闭路电视系统，对２０只Ｗｉｓｔａｒ大鼠肠系膜微淋巴管自律运动的动态过程进行了观察记录；应用淋巴微循环数字图象检测系统，实现了微淋巴管自律运动的频率特性、收缩指数和传播速度等待征参数的定量分析；从微血管自律运动和生物流体力学的原理出发，探讨了微淋巴管自律运动的传播特性、微淋巴瓣膜在自律运动中的作用机理以及微血管活性物质山莨菪碱对自律运动的影响。结果表明：微淋巴管自律运动沿管壁呈波浪式传播，是淋巴液单向连续流动的重要动力和调控因素；并推测微淋巴瓣膜对自律运动具有起搏样作用；实验发现山莨菪碱能够激活微淋巴管自律运动，加强淋巴微循环功能。

t is now generally accepted that the spontaneouscontraction of lymph vessel contributs to fluid and pro-tein transportation from the interstitial back to blood-strearn. In this study, 20 male Wistar rats with thepreparation of mesentery were divided into threegroups: valve group (6) , control group (7) , and 654-2group (7). We studied the mechanism of valve onrhythmical microlymphomotion and the effect of 654-2 ,a vasoactive substance, on lymphatic contractile activi-ty. The dvnarnic courses of contraction and relaxation ofperipheral rnicrolymphovessels were continuously visualized by a CCTV system. The frequency spectrum analysis of microlyrnphomotion (FC. AMP, and ANG) , thespreading speed of peristaltic wave (C mm/s) and thecontractile index (CI) were measured by using the new-ly developed microlymphomotion image processing system. The results dernonstrated that there were no sig-nificant differences between the frequency and amplrtudeof microlymphomotion existed above and below thevalves of microlymphovessel. There were also no signifi-cant changes in the spreading speed of peristaltic wave atthe up and down strearn measuring points crossing lym-phatic valve. But the contractile index crossing the valveincreased significantly (P< 0. 05 ). The down streamphase angle of microlymphomotion significantly led incornparision with that of upstrearn (P<0. 01 ). 654-2could activate and enhance lyrnphmotion which was in-hibited by NE. An energy conversion hypothesis oflymph flow and a Pacemaker hypothesis of microlym-phomotion were proposed.