肝素对大鼠肾小球系膜细胞的抑制作用

Inhibitory effect of heparin on glomerular mesangialcell

通过观察肝素对体外培养的大鼠肾小球系膜细胞（ＭｓＣ）生长、超微结构、细胞外基质合成及癌基因表达的影响，探讨肝素减轻以ＭｓＣ增生为特征的肾小球病变的可能性，分离培养的ＭｓＣ经鉴定后，分为肝素组和对照组进行观察，结果发现肝素可明显抑制ＭｓＣ生长，此作用具有剂量依赖性，电镜发现肝素使ＭｓＣ微丝减少，内质网和高尔基体扩张，次级溶酶体增多，髓样小体形成。免疫组化法证实肝素能抑制ＭｓＣ纤维连接蛋白和层联蛋白的合成，并抑制十四酚佛波乙酸酯（ＰＭＡ）刺激所致的ｃ～ｆｏｓ，ｃ－ｊｕｎ蛋白产物的表达。结合近年来肝素对ＭｓＣ抑制作用的体内研究，证实肝素对ＭｓＣ的增殖和代谢活性均有一定抑制性，为肝素治疗肾小球硬化性疾病提供了实验依据。

he pure mesangial cells (MsC) were isolated fromthe cuhure of rat glomeruli. After dentification, theywere divided into heparintreatment group and controlgroup. It was found that heparin profoundly inhibitedthe growth of MsC with 75. 9% inhibitory rate bymeans of direct counting MsC. MTT method provedthat the inhibitory effect of heparin on MsC was dose-dependent. Electronmicroscopic observation revealed di-lated endoplasmic reticulum and Golgi vesicles, moresecondary lysosomes, prominent myelin figures and decreased lmicrofilaments in the cytoplasm of the MsC ex-posed to heparin. Immunocytochemical studies showedthat the amounts of FN and LM in the cytoplasm ofMsC decreased after heparin treatment. Immunocytochemical investigation also confirmed that heparincould inhibite the expression of nuclear oncogene cfosand cjun in the MsC stimulated by PMA. The signifi-cance and possible mechanism of inhibitory effect of hep-arin on MsC were discussed.