细胞因子激活的巨噬细胞对细胞内利什曼原虫的杀伤性

Effects of cytokine-activated macropbages on intracel-lular leishmania

采用重组γ－干扰素（ＩＦＮ－γ）、α－肿瘤坏死因子（ＴＮＦ－α）和白细胞介素－３（ＩＬ－３）诱导巨噬细胞的活化，观察其对杜氏利什曼原虫的杀伤活性。结果，ＩＦＮ－γ（４００００Ｕ／Ｌ）处理巨噬细胞４８小时后，感染社氏利什曼原虫的巨噬细胞百分率为３４％，每个受染巨噬细胞内利什曼原虫无鞭毛体数平均为３．９个；而未经处理的对照组巨噬细胞，前者为６２％，后者平均为６．８个。两组数据差异有显著意义。ＩＦＮ－γ加ＴＮＦ－α（４００００Ｕ／Ｌ）处理巨噬细胞４８小时后，其巨噬细胞受染率仅为１６％，而每个受染细胞内平均原虫数目仅１．２个。但ＩＬ－３（１／１００）单独或与ＩＦＮ－γ联合均不能提高巨噬细胞的杀原虫作用。提示重组的ＩＦＮ－γ或ＴＮＦ－α均可激活巨噬细胞，两者联合对巨噬细胞的活化具有协同、增强作用。

he effects of activated macrophages on Leishmani-a donovani amastigotes and nitrite release weredetermined by in vitro inducement of macrophage acti-vation with recombinant IFN-r, TNF-α, and rIL-3.The results indicated that rIFN-r or rTNF-α at40 000U/L was able to induce a significant but modestlevel of macrophage activity, and synergistic effect ofrIFN-r and rTNF-α was apparent in the induction ofmacrophage activation. 48 hours after macrophageswere induced with both rIFN-r and rTNF-α, the per-centage of the macrophages infected by Leishmaniadonovani was 16 % , and the mean number of the parasite per infected macrophage was 1. 2±0. 04. There wasa strikingly significant difference in comparison with thecontrol group, in which the infection rate ofmacrophages was 62 % and the mean number of the par-asite per infected macrophage was 6. 8±0. 10. Howev-er, no significant difference was noted between rIL-3 at1/100 concentration, alone or combined with rIFN-r,and the control in leishmanicidal activrty ofmacrophages. An increase of in the culture super-natants was paralelled by the ability of various cytokine-activated macrophages to kill the intracellular parasites.