电场对神经再生微环境中活性蛋白的影响

Effect of electric fields on the protein in nerve regen-eration conditioned fluid

为探讨电场对神经再生的影响，作者采用神经再生室模型，将大鼠分四组：局部神经干电刺激组（Ｌ组）；脊髓神经元电刺激组（Ｎ组）；（失神经）肌肉电刺激组（Ｍ组）；对照组（Ｃ组）。于术后３、７、１４、２１、２８天，用微量进样器抽取神经再生条件液，进行１００×１０３分子量以下分子量区域的蛋白电泳与定量扫描分析。实验结果显示：Ｌ组在６．１６～１０．２３×１０３分子量区域，其蛋白含量显著高于其它组，其它组之间差异无显著性。认为此变化是电场促进神经再生的可能原因之一。

0 SD rats were randomly divided into four groupsof 20 each:local electrostimulation of nerve stump(Group LS) ; electro-stimulation of myeloneure (GroupN ); electrostimulation of the denervated muscle(Group M) ;and controls (Group C). The left lateralsciatic nerve of rats was excised smin in length, andthe severed nerve was bridged with the silicon tube.The gap between the sturnps was about 10mm. At3rd, 7th , 14th , 21st and 28th day after nerve transec-tion and tube implantation, the fluid in the silicon tubewas aspirated. The sample of fluid was spun and 4μlaliquots were taken from the supernatant. Then theelectrophoresis of the aliquots was made by phasesys-tem and assayed to the amount and variety by Gel Scansystem. The results showed that the group LS had ahigh increase in the protein amount than others in therange of 6. 16～10. 23 × 103 D molecular weight. Webelieve that it is one of the mechanisms of the electricfields promoting nerve regeneration.