氯化镍诱导DNA－蛋白质交联物的体内研究

An In Vivo Study DNA-protein Crosslinks Induced by Nickel Chloride

用氯化镍对大鼠腹腔注射染毒，以敏感的￣（１２５）Ｉ－后标记新技术检测了血白细胞（ＷＢＣ）和肺组织ＤＮＡ－蛋白质交联（ＤＰＣ）的形成情况。结果显示氯化镍急性染毒后引起ＷＢＣ和肺ＤＰＣ明显升高并有剂量反应关系；小量多次染毒的结果也与１次染毒相一致。表明ＤＰＣ能反映镍化合物对ＷＢＣ和肺器官的遗传毒性，可作为毒作用分子生物标记。结果中还发现白细胞ＤＰＣ的升高较明显并与肺ＤＰＣ有相关性。提示白细胞ＤＰＣ可作为靶器官的替代物反映镍对靶器官的遗传损害。

NA-protein crosslinks (DPC) in the white blood cells (WBC) and lung tissues were detected with anew and sensitive￣(125) I-post-labelling technique in male Sprague-Dawley rats injected peritoneally(i.p.)with nickel chioride (NiCl_2).Results showed DPC in the WBC and lungs of the rats increased signifi-cantly 20 hours after acute exposure to NiCl_2.Multiple small doses( 10mg/kg i.p.,twice a week for three weeks )of NiCl_2 had similar effects as single large dose(30 mg/kg ).It suggested DPC could be used as a bioma rker to reflect genotoxic ity to the WBC and lungs caused by nickel compound,DPC in the WBC increased more obviously than that in the lungs after exposure to NiCl_2,and both correlated to each other.It showed DPC in the WBC can be used as a surrogate to indicate genotoxic lesions in target organs.