摘要
将ＳＤ大鼠９０只随机分体内和体外实验组，体外实验又分为假伤，烫伤和ｒｈＴＮＦ－α治疗组，观察重组人肿瘤坏死因子－α（ｒｈＴＮＦ－α）对烫伤大鼠Ｔ淋功能的影响。结果表明，小剂量静脉注射ｒｈＴＮＦ－α可以改善烫伤大鼠脾脏Ｔ淋巴细胞亚群的分布，并使Ｔｈ／Ｔｓ比值高（Ｐ＜０．０５），Ｗ３／２５（Ｔｈ）数量增加，ＯＸ－８（Ｔｓ）数量减少；体外实验表明，ｒｈＴＮＦ－α可以改善Ｔ淋巴细胞对ＣｏｎＡ的反应能力。
SD rats(200～230g body weight)were randomly divided into three groups:①sham burn;②burn;③burn plus rhTNF-α.Thirddegree burn of 30% TBSA on the trunk was produced in groups 2 and 3.Intravenous rhTNF-α(10μg/kg)was ad- ministered 6 hours postburn and once daily thereafter for 5 days in group 3.At 5 days postburn,splenic lymphocytes obtained from all the animals were used for the determi- nation of distribution of T lymphocyte subsets with flowcytic analysis and activity of IL- 2(Gills method).The results indicated that low dose rhTNF-a in vivo could improve dis- tribution of splenic T lymphocyte subsets in burned rats by increasing the numbers of W3/25(Th)and decreasing the number of OX-8(Ts) ,and the ratio of W3/25/OX-8 was raised.In vitro the proliferation response of T lymphocytes to ConA was enhanced and the activity of IL-2 was increased by 108%,149% and 168%,respectively,in group 3 compared with that in group 2.However,a dose-dependent effect,in that a small dose (≤10 ng/2.5×10~6 cells)of rhTNF-αimproved the immune function of T lymphoeytes, while a higher dose inhibited T lymphocyte immune function.These findings showed that rhTNF-α improved not only the distribution of T lymphocyte subsets but also their immune activity.
