摘要
采用 ATPase 染色法对人皮、豚鼠皮分别进行常温新鲜皮片组及 H 抗冻液组郎格罕氏细胞(LC)计数的比较,并对4种(4℃,-20℃,-80C,-196℃)不同温度处理组的6个(1,2,3,5,7,14天)时相点的 LC 进行了动态观察。结果表明,人皮及豚鼠皮常温新鲜皮片组与 H 抗冻液皮片组的 LC 计数,在数量与形态学方面比较差异均无显著意义(P>0.05)。不同温度处理组及其各时相点 LC 计数规律为:冷冻温度越低,各实验组相同时相点的 LC 均值下降越显著,LC 下降速率的谷值,以-196℃组下降最快(1天),该冷冻组降低的幅度也最大(仅为正常值的40%～50%)。在细胞形态学观察中发现冷冻后 LC 均存在明显变形、胞体缩小、胞突变短、消失等结构的改变。皮肤表皮 LC 是重要的移植抗原,它的数量的减少及细胞形态改变可能将会对推迟异体皮片的排斥反应及延长皮片移植后存活时间起到重要作用。
The skin from fresh human cadavers and guinea pigs was stored in 4 C, —20℃,—80℃and—196℃ deep freezing for six different periods(1,2,3,5,7 and 14 days).The number and morphology of Langerhans ceils(LC)were observed using Juhlin's ATP—ase dye method.It was found that the epidermal LC of cryopreserved skin decreased significantly in number,and there was a marked change in shape,as com- pared with fresh skin grout(P<0.01),except for those in 4C groups(for 1,2,3 days). The mean value of LC in —196℃ group,which was 40%～50% of that of fresh skin, was the lowest among all the groups.The number of LC dropped most rapidly in the —196℃ group after one day freezing.The changes in LC in the stored skin may play an important role in prolongation of rejection time after transplantation.
