摘要
采集了70例烧伤病人129份外周血中性粒细胞(PMN),以O_2、特殊颗粒(SG)和胞内杀菌力(ICBA)为指标动态观测了深度烧伤后病人的PMN水平变化;分析了病人血浆对正常人PMN胞内指标的有害作用,及抗人C_3、C_3血清(AHC_3C_3S)对上述有害作用的特异阻断效能。结果表明:①重、中度烧伤病人的各指标值均显著低于正常水平(P<0.05);当BSD(深度烧伤面积)≥45％和病程在1～6天时,ICBA最低。②1CBA与SG和O_2的动态相关显著(P<0.05)。③病人血浆能显著削减正常人PMN胞内1CBA、SG和O_2^-的储存,而AHC_3C_S能减轻这些储存的丢失,效能由保护率表示;ICBA(67.33％)>SG(51.60％)>O_2^-(46.68％)。结果提示:PMN杀菌力的高低与BSD和病程早晚呈负相关,C_3、C_5碎片是PMN胞内ICBA下降的重要直接因素。
129 PMN-samples from the peripheral blood of 70 burnt patients were collected, and ICBA, SG and O_2^- were dynamically studied. The harmful effects on patients' plasma on normal human PMN, and specific blocking effect of anti-human C_3. C_5 serum (AHC_CS)on the above ha harmful effects were also observed. The results:①All the parameters values showed a significant decrease in seriously or moderately burnt pa- tients as compared with normal values; the decrease was most marked on 1 st-6 th postburn days when deep burn surface exceeded 45%. ②ICBA was significantly corre- lated with SG and O_2^-.③Patients' plasma greatly reduced the reserves of ICBA, SG and O_2^- in normal PMN, while AHC_3C_5S might lessen the reduction in term of net reserve rates: ICBA (67.33)>SG(51.60)>O_2^-(46.68). The findings suggest: ICBA levels are reversely related with DBSA and fragments of C_3 and C_5 are the main factors in reduction of ICBA.
关键词
烧伤
补体
胞内杀菌力
超氧离子
Complement
Specific granules: Intracellular bactericidal activity
Burn
