透明质酸刺激因子对深II度烧伤创面瘢痕形成的影响

Effect of hyaluronic acid-stimulating factor on scar formation in wound healing process of deep partial thickness burns

从兔羊水及胎兔血清中提取透明质酸刺激因子(HASF)液。将其用于深Ⅱ度烧伤创面,采用自身对照法,通过观测早期创面渗液中透明质酸(HA)含量变化、愈合后一个月局部组织的外观及镜下观和组织中胶原含量的分析,从中探讨了HASF在深Ⅱ度烧伤创面愈合过程中对瘢痕形成的影响。此外还将HASF用于体外培养的兔成纤维细胞,观测上清液中HA含量的改变。结果表明,HASF可以明显提高创面渗液中HA的含量,体外实验也证实HASF有促成纤维细胞产生HA的作用,而HA具有调节胶原合成的作用,可提高Ⅲ型胶原的比率,使胶原纤维变细,从而减轻瘢痕的产生。此外发现,在瘢痕组织中,Ⅰ、Ⅲ型胶原总量和正常皮肤间差异无显著意义(P>0.05)。

Hyaluronic acid-stimulating factor(HASF) was purified from the amni- Otic fluid and fetal serum of the gestational rabbit by means of affinity chromatography. After topical application of HASF on the deep partial thickness burn wounds, the level of hyaluronic acid (HA) in wound exudate was determined daily during the early period of healing. One month after wound healing, the local skin was harvested for collagen analysis. Histologic study was made to check the outline of collagen fibrils. The HASF was also used in rabbit fibroblast culture. The HA level contained in the supernatant was assayed. Result showed that HASF could elevate and maintain the HA level in the extracellular matrix of burn wound. In vitro, HASF was able to stimulate the fibroblast to produce hualuronic acid. The presence of HA in extracellular matrix then modulated collagen synthesis in the process of wound repair resulting in a higher content of type III collagen, thus bringing about a scarless healing of the deep patrial thickness burns.