摘要
利用小鼠3T3成纤维细胞具有促进人表皮细胞生长的特点,观察了无血清培养时3T3细胞对低密度接种的传代表皮细胞生长的影响。结果表明表皮细胞具有较高的生长能力,细胞融合时间明显缩短,传代比例和培养面积明显扩大,这对临床大面积烧伤病人的救治具有重要意义。
We propose a modification of the conventional keratinocyte subculture method,showing a significant improvement in the colony growth capacity of subcultured keratinocytes.This method utilized mouse 3T3 fibroblasts and MCDB153 medium,and it has been shown that they could improve the colony growth of human keratinocytes. Compare with the conventional procedure,this culture method was shorter in time,and resulted in a higher colony growth capacity of cells ,reaching confluence 3 days earlier.In the treatment of extensively burned patients using cultured epidermal sheets,the time required for their production remains the main problem.Thus,the significantly reduced time to obtain confluent keratinocyte sheets with our method is very important for the treatment of large burn wounds.
基金
国家自然科学基金资助课题
