摘要
作者采用荧光染色法检测，证实人胃癌ＳＧＣ－７９０１细胞具有雌激素受体。在细胞培养过程中予以不同浓度的雌二醇处理，结果显示：于０．００１～１μｍｏｌ／Ｌ浓度时可刺激ＳＧＣ－７９０１细胞的生长，其中以０．１μｍｏｌ／Ｌ刺激作用最强，细胞群体倍增时间短于对照组（Ｐ＜０．０５），克隆形成率明显高于对照组（Ｐ＜０．０１），ｃ－ｍｙｃ癌基因的表达亦较对照组强。流式细胞术检查发现，经雌二醇处理后，细胞增殖指数略高于对照组，而雌激素受体抑制剂三苯氧胺，可部分抑制雌二醇所产生的上述这些效应。实验结果提示，生理浓度的雌二醇对ＳＧＣ－７９０１细胞具有一定的生长刺激作用，而三苯氧胺可部分抑制这种刺激作用。其机制可能是通过雌激素受体介导的。
Abstract In this study,the effect
was studied of estradiol(E_2)and tamoxifen on the growth of a hu- man
gastric adenocarcinoma SGC-7901 cell line in vitro. Estrogen receptor
as revealed by immunohistochemical staining was positive. when added
at various concentrations.E_2 could significantly stimulate cell
growth.The effect was most marked at concentration of 0.1 μmol / L.
The cell doubling time was reduced,the plating efficiency was
increased and c-myc oncogene expression was enhanced. Flow cytometric
analysis indicated that cell proliferation index was greater in the
E_2-treated than in the control.All these effects caused by E_2 could
be partially inhibited by the estrogen receptor inhibitor tamoxifen.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1995年第2期93-96,共4页
Chinese Journal of Oncology
