摘要
用 ̄(125)I标记的人类表皮生长因子( ̄(125)I-EGF)与分离制备的结(白)肠癌和癌旁正常组织细胞膜进行放射配体结合试验。结(直)肠癌细胞膜 ̄(125)I-EGF结合量为20.16±4.76fmol/mg膜蛋白,癌旁正常组织为11.22±2.96fmol/mg膜蛋白,两者差异有显著性意义(21例,t=7.33,P<0.01)。Scatchard分析表明,结(直)肠癌细胞膜EGF受体为单一亲和力的受体。结(直)肠癌细胞膜与 ̄(125)IEGF的最大结合量(Bmax=38.07±4.75fmol/mg膜蛋白)高于癌旁正常组织(Bmax=29.31±164fmol/mg膜蛋白),两者差异有显著性意义(t=2.97,P<0.05)。结(直)肠癌EGF受体的亲和力为K_D=1.31±0.33nmol/L,大于癌旁正常组织(K_D=1.34±0.40nmol/L),但两者间差异无显著性意义(t=0.1,P>0.05)。研究结果表明,结(直)肠癌细胞膜表面EGF受体数量有显著改变,提示在肿瘤的发生发展过程中,EGF与其受体所形成的自/旁分泌增殖环起着重要的生长调节作用。
Abstract Membrane extracts of surgically resected colorectal
cancer and nearby non-cancerous tis-sues were prepared and the
expression of epidermal growth factor receptor(EGF-R)was exam-ined by
 ̄(125)I-EGF binding assay. Scatchard analvsis indicated that a
single class of EGF-Raspresent in the cancer as well as the
non-cancerous tissues.EGF-R was found over-expressedin cancer(38.07
±4.75 fmol/mg protein)as compared to that in the
non-canceroustissue(29.31± 1.64 f mol / mg protein)(P<0.05).However,
the difference in binding affinitiesof EGF-Rin the cancer and
non-cancerous tissues was not statistically significant. The
resultssuggest an important role of an autocrine / paracrine loop of
growth regulation in colorectal cancer.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1995年第2期89-92,共4页
Chinese Journal of Oncology