摘要
用￣（１２５）Ｉ标记的人类表皮生长因子（￣（１２５）Ｉ－ＥＧＦ）与分离制备的结（白）肠癌和癌旁正常组织细胞膜进行放射配体结合试验。结（直）肠癌细胞膜￣（１２５）Ｉ－ＥＧＦ结合量为２０．１６±４．７６ｆｍｏｌ／ｍｇ膜蛋白，癌旁正常组织为１１．２２±２．９６ｆｍｏｌ／ｍｇ膜蛋白，两者差异有显著性意义（２１例，ｔ＝７．３３，Ｐ＜０．０１）。Ｓｃａｔｃｈａｒｄ分析表明，结（直）肠癌细胞膜ＥＧＦ受体为单一亲和力的受体。结（直）肠癌细胞膜与￣（１２５）ＩＥＧＦ的最大结合量（Ｂｍａｘ＝３８．０７±４．７５ｆｍｏｌ／ｍｇ膜蛋白）高于癌旁正常组织（Ｂｍａｘ＝２９．３１±１６４ｆｍｏｌ／ｍｇ膜蛋白），两者差异有显著性意义（ｔ＝２．９７，Ｐ＜０．０５）。结（直）肠癌ＥＧＦ受体的亲和力为Ｋ＿Ｄ＝１．３１±０．３３ｎｍｏｌ／Ｌ，大于癌旁正常组织（Ｋ＿Ｄ＝１．３４±０．４０ｎｍｏｌ／Ｌ），但两者间差异无显著性意义（ｔ＝０．１，Ｐ＞０．０５）。研究结果表明，结（直）肠癌细胞膜表面ＥＧＦ受体数量有显著改变，提示在肿瘤的发生发展过程中，ＥＧＦ与其受体所形成的自／旁分泌增殖环起着重要的生长调节作用。
Abstract Membrane extracts of surgically resected colorectal
cancer and nearby non-cancerous tis-sues were prepared and the
expression of epidermal growth factor receptor(EGF-R)was exam-ined by
￣(125)I-EGF binding assay. Scatchard analvsis indicated that a
single class of EGF-Raspresent in the cancer as well as the
non-cancerous tissues.EGF-R was found over-expressedin cancer(38.07
±4.75 fmol/mg protein)as compared to that in the
non-canceroustissue(29.31± 1.64 f mol / mg protein)(P<0.05).However,
the difference in binding affinitiesof EGF-Rin the cancer and
non-cancerous tissues was not statistically significant. The
resultssuggest an important role of an autocrine / paracrine loop of
growth regulation in colorectal cancer.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1995年第2期89-92,共4页
Chinese Journal of Oncology
