摘要
用ＨＩＬ系列单抗分别筛选了由高转移Ａｎｉｐ［９７３］细胞亚系及其母系低转移ＡＧＺＹ－８３ａ细胞系构建的ｃＤＮＡ表达文库。从Ａｎｉｐ［９７３］ｃＤＮＡ文库中筛选出一个阳性克隆（Ｈ＿４－Ｄ），经序列分析其长度为９７８ｂｐ，开放阅读框架为３１８ｂｐ，编码１０６个氨基酸，与人丙酰辅酶Ａ羧化酶α链有８５％的同源性。Ｗｅｓｔｅｒｎｂｌｏｔ分析显示Ｈ＿４单抗识别Ａｎｉｐ［９７３］细胞膜蛋白分子量为１５ＫＤ和２７ＫＤ的两条带。Ｈ＿４－Ｄ在Ａｎｉｐ［９７３］细胞中的表达高于在ＡＧＺＹ－８３ａ细胞。Ａｎｉｐ［９７３］细胞经Ｈ＿４单抗预处理后，转移能力明显降低。以上结果表明，Ｈ＿４－Ｄ与Ａｎｉｐ［９７３］细胞的转移表型有某种程度的相关性。
Abstract
The cDNA expression libraries derived from a highly metastatic cell
subline Anip[973]andfrom the poorly metastatic , parental cell line ,
AGZY-83a were screened by monoclonal antibodiesHIL. A positive
clone(H_4-D) from the Anip[973] cDNA library was isolated and its
nucleotidesequence was determined.This clone contained 978 bp with an
open reading frame of 318 bp en-coding a polypeptide consisting of
106 amino acids. The H_4-D cDNA sequence showed 85%ho-mology with a
human propiony-CoA carboxylase α-chain. In Western blotting
analysis,theMoAb H_4 recognized 2 bands(15KD and 27KD ) of Anip[973]
cell membrane proteins.ThemRNA expression of H_4-D was higher in
Anip[973] cells than in AGZY-83a cells.The metastaticpotential of
Anip[973] cells was markedly decreased after being pretreated with
MoAb H_4.Theabove findings indicate that H_4-D has a certain
relationship with the metastatic phenotype ofAnip[973] cells.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1995年第3期163-166,共4页
Chinese Journal of Oncology
基金
国家自然科学基金
