荧光原位杂交技术检测外周T细胞淋巴瘤的染色体畸变

DETECTION OF CHROMOSOME ABERRATIONS IN PERIPHERAL T-CELL LYMPHOMAS BY FLUORESCENCE IN SITU HYBRIDIZATION

运用荧光原位杂交技术，在４１例外周Ｔ细胞淋巴瘤（简称ＰＴＬ）中发现３２例（７８％）和１４例（３４％）病人分别有常见的第３号染色体三体（＋３）以及一个额外Ｘ染色体（＋Ｘ）畸变，含有＋３和＋Ｘ的畸变细胞率分别为４．５％和８．２％。研究表明，＋３和＋Ｘ是ＰＴＬ常见的、且可能是特异性的染色体畸变，恶性肿瘤细胞在受累淋巴结中只占少数，荧光原位杂交技术在检测染色体畸变中比传统的细胞遗传学方法敏感。

AbstractUsing fluorescence in situ hybridization (FISH)technique,we have detected trisomy 3(+)and gain of an extra X chromosome(+X)in 32(78%)and 14(34%)of 41 patients with periph-eral T-cell lymphomas,respectively.However,cells with trisomy 3 and +X were,on average,4.5%and 8.2%, respectively.The study showed that trisomy 3 and +X were the specific chro-mosome aberrations of peripheral T-cell lymphoma;cells with+3 and +X constituted only asmall part in a specimen:FISH was more sensitive than traditional cytogenetics in detection ofchromosome aberrations.