nm23－H1等位基因缺失与大肠癌转移相关性研究

CORRELATION STUDY OF ALLELIC GENE DELETION OF nm23-H1 AND HUMAN COLORECTAL CARCINOMA METASTASIS

ｎｍ２３基因是近年来发现的与肿瘤转移表型抑制相关的基因，称之为转移抑制基因。ｎｍ２３基因的等位基因缺失、突变和低表达与多种人类肿瘤转移相关。ｎｍ２３基因包括ｎｍ２３－Ｈ１及ｎｍ２３－Ｈ２两种亚型。我们利用Ｓｏｕｔｈｅｒｎ杂交技术检测了２３例大肠癌及其相应正常粘膜基因组ＤＮＡｎｍ２３－Ｈ１等位基因缺失情况，结果发现５例存在ｎｍ２３－Ｈ１等位基因缺失。有淋巴结、肝或其它脏器转移者，ｎｍ２３－Ｈ１等位基因缺失率为５７．１％（４／７），而无转移者为６．２％（１／１６）。两组比较差异有显著性意义（Ｐ＜０．００５）。ｎｍ２３－Ｈ１等位基因缺失与肿瘤大小、部位及分化程度无显著相关（Ｐ＞０．０５）。结果表明，ｎｍ２３－Ｈ１基因在抑制大肠癌转移方面起重要作用。

AbstractIn recent years, a tumor suppressor gene nm23 has been found to be associated with decreasedtumor metastatic potential. Allelic deletion ,mutation and low expression of this gene has been corre-lated with tumor metastatic potential in a number of tumors. There are two known isotypes of humannm23 gene,named nm23-H1 and nm23-H2. We examined DNA from 23 cases of colorectal carcino-mas and their corresponding normal mucosa using Southern blot hybridization with nm23-H1 cDNAprobe. Five cases with allelic deletion of nm23-H1 gene were found,with allelic deletion rate of 57.1%(4/7) in cases with metastasis to lymph node,liver or other organs, and 6.2%(1/16)in caseswithout metastases (P<0. 005).There is no correlation between allelic deletion and tumor siz loca-tion or differentiation. This result indicates that nm23-H1 gene plays an important role in the metas-tasis of colorectal carcinoma.