外源性IL－6对人脑髓母细胞瘤生长的促进作用

GROWTH PROMOTING EFFECT OF IL-6 ON MEDULLOBLASTOMA CELLS IN VITRO

作者采用分子生物学和细胞免疫学手段，在国内外首次研究了ＩＬ－６基因在髓母细胞瘤内的表达和产生，及其对肿瘤细胞生长的作用。结果发现，１３例被检样本中１２例有ＩＬ－６信息传导基因─ＩＬ－６Ｒ和ｇｐ１３０的表达，但无一例表达ＩＬ－６。重组ＩＬ－６可明显促进细胞生长和ＤＮＡ合成。结果提示，ＩＬ－６可通过旁分泌途径促进髓母细胞瘤生长。由于髓母细胞瘤周围和内部存在ＩＬ－６分泌细胞，本研究对揭示肿瘤与其间质细胞间的相互作用有一定的理论和临床意义。

Abstract Expression of IL-6 and IL-6 signal transducer genes(IL-6R and gp130)in human medulloblas-toma cells were investigated by Northern blot analysis,RT/PCR and immunocytochemical staining.The results revealed that among 13 tumor cases and 3 established medulloblastoma cell lines, 95%ofthem were found to express full set of IL-6 signai transducer genes, IL-6R and gp 130, but none ofthem expressed IL-6. Incubation of medulloblastoma cells in vitro with recombinant IL-6 could appar-ently stimulate cell growth and enhance DNA synthesis. Our data thus for the first time demonstratethat though IL-6 is not expressed by medullObiastoma cells themselves, IL-6 can act on medulloblas-toma cells through its signal transducers by a paracrine pathway. Since medulloblastomas are sur-rounded by gliosis and infiltrated by immune activated cells which are known to express IL-6 , the in-fluence of those IL-6 producing cells on the development and progression of hiedulloblastomas shouldbe taken into account.