食管癌和癌旁上皮中乳头状瘤病毒的检测

DETECTION OF HUMAN PAPILLOMA VIRUS IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA AND ADJACENT TISSUE SPECIMENS IN LINXIAN

作者从河南林县收集了８７例外科手术的食管标本，包括３５例食管鳞状上皮癌及５２例食管癌旁上皮（肉眼未见肿瘤）。用Ｓｏｕｔｈｅｒｎｂｌｏｔ法（ＨＰＶ１６与１８型探针）和ＰＣＲ方法（ＨＰＶ的公共引物）分析了上述标本中ＨＰＶＤＮＡ的存在，并以胎儿食管上皮ＤＮＡ和外阴癌ＤＮＡ（含ＨＰＶ６型）作为对照。结果显示：Ｓｏｕｔｈｅｒｎｂｌｏｔ与ＰＣＲ方法未检查出任何一例食管癌或癌旁上皮组织中有ＨＰＶ１６型、１８型和其它类型ＨＰＶＤＮＡ的存在。因此，ＨＰＶ在食管癌发生发展中的作用尚未明确。林县食管癌的高发病率可能和其它因素如亚硝胺有关。

Abstract A total of 87 human esophageal tissue specimens,comprising 35 squamous cell carcinomasand 52 adjacent tissue specimens(with no gross evidence of tumors)were obtained from patientswho underwent surgery for cancer of the esophagus in Lin-xian county,Henen Province.The tis-sue specimens were analyzed for the presence of HPV DNA by Southern blot assay with HPVtype 16 and 18 probes and by polymerase chain reaction(PCR)with HPV consensus primer. Theesophageal epithelial specimens of human fetus,and condyloma tissue contained HPV type 6 wereused as controls.The results showed that no HPV 16,18 and other type were detectable in anycase,either in the esophageal carcinomas or adjacent tissue specimens.Thus,the role of HPV inthe carcinogenesis of esophagus remains unclear.The high incidence of esophageal squamous cellcarcinoma in Lin-xian is probably related to other factors such as N-nitrosamines.