人胎儿脾脏T淋巴细胞的发育

DEVELOPMENT OF T LYMPHOCYTES IN FETALSPLEEN OF HUMAN

应用抗Ｔ淋巴细胞单克隆抗体，采用间接免疫荧光法，对人胎儿脾脏Ｔ淋巴细胞表面抗原表达进行了测定。结果显示，１６周胎脾中ＣＤ＿３＋细胞为２５．５±３．７％，２０周时达３５．０％～４２．６％；１６～３２周胎脾中ＣＤ＿４＋细胞／ＣＤ＿８＋细胞比值随胎龄而增加；ＣＤ＿（３８）＋细胞为７１．０％～８８．０％，ＣＤ＿１＋细胞为１２．０％～１５．０％；ＣＤ＿３＋细胞与ＣＤ＿２＋细胞百分比基本一致。对Ｔ淋巴细胞发育的有关问题进行了讨论。

The monocbnal antibodies against T lymphocytes have been used to identify T lymphocytes phenotype in human fetal spleen using indirect immunofluorescence tech- nique. The results showed that in spleen suspensions of 16 week old fetuses CD_3+cells were 25.5±3. 76%and CD_3+cells were 35.0%～42.6%in spleen suspensions of 20～32 week old fetuses. The ratioes of CD_4+ cells/CD_8+ cells were increase with fetal ages.In spleen suspensions of 16.0%～3.0%week old fetuses about 71.0%～88.0%cells were CD_(38)+cells, 12.0%～15.0%cells were CD_1+cells. The percentages of CD_3+and CD_2+T-cells were almost equal in the spleen suspensions of l 6～32 week old fetuses。The problems about developments of T lymphocytes were discussed.