薄层扫描法测定三灵通脉口服液中人参皂甙Rb_1含量

TLC SCANNING DETERMINATION OF GINSENOSIDE RB_1 IN SAN LING TONG MAI ORAL LIQUID

采用大孔吸附树脂－薄层扫描法测定三灵通脉口服液中人参皂甙Ｒｂ＿１含量。展开剂：氯仿－甲醇－水（６５：３５：１０）。显色剂：１０％乙醇硫酸溶液。扫描条件：λ＿ｓ＝５３５，λ＿Ｒ＝６８０，ＳＸ＝５。线性范围：０～１０μｇ，γ＝０．９９９４。平均回收率９９．１±３．０％。结果表明，本方法回收率好、分离率高，可作为本制剂的含量测定方法。

Quantitative determination of ginsenoside Rb_1 in San Ling Tong Mai oral liquid was done by macroporous absorbing resin-TLC scanner,Developer:CHCl_3:CH_3OH:H_2O(65:35:10).Color developing agent:10%H_2SO_4 in ETOH.Scanning condition:λ_s= 535 nm,λ_R=680 nm, SX=5.Linear range:0～10μg,r=0.9994.Average recovery 99.1±3.0%.The results showed that the recovery is good and the resolution is high. This method can be used for measuring ginsencoside Rb_1 in this preperation.