人脑海马内部微血管三维构筑的研究

EFFECT OF MONOCLONAL ANTIBODY ON LAK CELLS ACTIVITY AGAINST PANCREATIC CARCINOMA

本文收集广州地区６个月胎儿至６岁儿童新鲜大脑标本６３例（１２６侧），其中５例作脑血管有机玻璃单体铸型，３５例用碱性磷酸酶染色（包括７例墨汁灌注后复染），其余用于墨汁灌注法，在光镜暗视野全景放大观察和日立Ｓ－４５０扫描电镜观察，较完整地显示人脑海马内部血管动脉－微动脉－毛细血管前括约肌－毛细血管－微静脉－小静脉连续性立体构筑。此外还观察到微血管形态特征及微血管间存在多种吻合。

he purpose of this study was to investigate the efficacy of anti-pancreatic carcinoma of lymphokine activated killer cells (LAK cells ) mediated by YPC3 monoclonal antibody (YPC3 mAb) in vitro and vivo. In 4hr 51Cr release assays, the cytolysis of Capan-2 human pancreatic carcinonma cells by LAK cells was enhanced by pancreatic carcinoma specific YPC3 mAb. This antibody-dependent cellular cytotoxicity (ADCC) of the LAK cells was more evident while increasing the concentration of YPC3 mAb. When 50ug/ml of YPC3 mAb was used the cytotoxic effects of LAK cells on target cells increased about 60%. No cytotoxic effect of the LAK cells was found in the presence of irrelevent monoclonal antibody. Experimentally, the growth rate of Capan-2 human pancreatic carcinoma cell line in nude mice was 25%, 100% and 100% after the injection of LAK cells , splenocytes and YPC3 mAb respectively. Howev- er, simultaneous injection of YPC3 mAb and LAK cells completely inhibited the growth of the cell line. These results suggest that LAK cells in combination with YPC3 mAb might be use- ful for the treatment of human pancreatic carcinoma.