PCR法检测耐甲氧苯青霉素金黄色葡萄球菌的mecA基因

RAPID GENETIC DIAGNOSIS OF THE DELETED -TYPE MUSCULAR DYSTROPHY BY MULTIPLEX POLYMERASE CHAIN REACTION

临床标本进行ＤＮＡ抽提后，用聚合酶链反应（ＰＣＲ）将具有６２３个ｂｐ的耐甲氧苯青霉素金黄色葡萄球菌（ＭＲＳＡ）的ｍｅｃＡ基因扩增，经琼脂糖电泳及ＤＮＡ印迹杂文（ｓｏｕｔｈｅｒｎｂｌｏｔｈｙｂｒｉｄｉｚａｔｉｏｎ），并用ＥＣＬ３’寡核苷酸标记增强化学发光进行检测，其结果与ＭＲＳＡ常规培养方法作比较。结果显示，７３份临床标本中，１５份常规培养法ＭＲＳＡ和ＰＣＲ法ｍｅｃＡ基因均为阳性，另１份ｍｅｃＡ基因扩增阳性的标本，常规培养ＭＲＳＡ为阴性。作者认为，由于ＰＣＲ对检测ＭＲＳＡ的ｍｅｃＡ基因具有快速、较强的特异性和敏感性等特点，作为检测临床标本中的ＭＲＳＡ的方法是可行的。

AbstractOne or more fragment has been found to be deleted in five DMD and two BMD patients by multiplex polymerase chain reaction in a survey of ten muscular dystrophy pedigrees in which thirteen males are affected (only two BMD in a single pedigree). The genomic DNA of all the affected patients have been analyzed by multiplex PCR involving a two-step method in which five fragments were firstly amplified whereas the next four in another absolute eppen- dorf later. Our results revealed that exon 45 and exon 48 are the more frequent ones involved in the events of deletions in the dystrophin gene. With these results we can take a direct and accurate pregnant diagnosis in these five deletion-positive fa