经化疗药物作用的肿瘤细胞对LAK细胞敏感性研究

STUDY ON SYNERGETIC EFFECT OF LAK CELL AND ANTITUMOR DRUGS

应用肺腺癌细胞株Ａ５４９作为靶细胞，探讨ＬＡＫ细胞与抗癌药物协同杀伤肿瘤细胞的作用。浓度为１０μｇ／ｍｌ～０．１５５μｇ／ｍｌ的丝裂霉Ｃ、阿霉素、顺铂３种抗瘤药物作用于Ａ５４９细胞２４或４小时以后，再加入ＬＡＫ细胞并用乳酸脱氢酶释放法测定细胞毒作用。结果显示，丝裂霉素不改变细细胞对ＬＡＫ细胞的敏感性、阿霉素的作用可随浓度增加使ＬＡＫ细胞对肿瘤细胞的杀伤作用增强。而顺铂则在特定浓度显示与ＬＡＫ细胞有协同作用。

Using the lung adenocarcinoma cell line A549 as targeted cell,we have studied the synergetic effect of LAK cell and three antitumor druges:MMC,ADM,CDDP.A549 cell was treated with the three different drugrs for 4 or 24 hours in a series of concentrations from 10μg/ml to 0.155μg/ml by seven half dilution,and then the cytotoxity of LAK cell to targeted cell was measured by LDH release.The results show that MMC has no effect on sensitivity of A549 cell to LAK cell,ADM can increase the sensitivity of A549 related to its concentration,CDDP and LAK cell have obvious synergetic effect,which is related to the special concentration of CDDP.