广西抗－HCV阴性肝癌病人血清及肝癌组织丙型肝炎病毒核酸检测

DETECTION OF HEPATITIS C VIRUS RNA IN ANTI-HCV-NEGATIVE SERA AND LIVER TISSUES FROM THE PATIENTS WITH HEPATOCELLULAR CARCINOMA IN GUANGXI,CHINA

１９９２年曾用Ａｂｂｏｔｔ公司第二代酶免疫试剂盒检测１８６例广西肝癌病人抗－ＨＣＶ抗体，结果阳性率仅５．４％，但抗－ＨＣＶ阴性并不能排除丙型肝炎病毒感染，为了更确切地了解广西肝癌病人中的丙肝病毒感染状况，本研究用聚合酶链反应技术（ＰＣＲ）对５９例抗－ＨＣＶ阴性肝癌病人血清及１７例石蜡包埋肝癌组织进行了丙肝病毒核酸（ＨＣＶＲＮＡ）检测，结果５９例血清中检出ＨＣＶＲＮＡ阳性６例（１０．２％）．但１７例肝癌组织中无１例检出ＨＣＶＲＮＡ。又用ＰＣＲ方法检测这１７例肝癌组织之ＨＢＶＤＮＡ，结果７例显示阳性。以上结果表明，抗－ＨＶＶ加检ＨＶＶＲＮＡ可以提高丙肝病毒感染的检出率。尽管如此，与其他国家和地区相比，广西肝癌中总的丙肝病毒感染率（估计为１５．０％）仍然是较低的。

In 1992,we measured the anti-hepatitis C virus antibody (anti-HCV) of 186 patients from Guangxi province with hepatocellular carcinoma (HCC) by using second-generation Abbott enzyme-linked immunoassay kits and found the anti-HCV,prevalence was considerablly low (only 5.4%).Considering the negative anti-HCV can not exclude the presence of HCV infection and in order to mor accurately assess the relationship of hepatitis C virus to HCC,in this study we examined HCV RNA in the sera of 59 anti-HCV-negative HCC patients and paraffin-embedded HCC liver tissues from other 17 patients by using nested polymerase chain reaction (PCR),As a result,HCV RNA was detected in the sera of 6 out of 59 (10.2%) HCC patients but in none of liver tissue samples from 17 HCC patients.PCR was also performed on these liver samples for hepatitis B virus DNA and the result showed that 7 of them were positive.Our results indicate measurement of anti-HCV as well as HCV RNA can increase the detected rate of HCV infection,Even so,the total prevalence of HCV infection in HCC patients of Guangxi is still low (15.0% in estimation).It implies that the role of hepatitis C virus,compared with hepatitis B virus,in the development of HCC in Guangxi is smaller;