藻蓝蛋白（phycocyanin）─铜激光对大肠癌细胞株HR─8348的杀伤效应

The photodynamic effect of the phycocianin with a pulsed copper vapour laser on the human colorectal cancer cell line HR8348

大肠癌细胞株ＨＲ－８３４８用含有１００ｕｇ／ｍｌ，５０ｕｇ／ｍｌ和２５ｕｇ／ｍｌ（藻蓝蛋白１６４０培养液处理后，用波长为６３０ｎｍ的脉冲铜激光照射细胞１２Ｊ／ｃｍ２，继续培养２４小时后用ＭＴＴ法测得细胞存活率为２２．１％，３７．６％和８９．７％，显示出一定的剂量效应，与市售光敏剂蚕砂叶啉对比观察，证明其杀伤肿瘤细胞的效力优于蚕砂叶啉。实验表明藻蓝蛋白有明显的光敏效果。藻蓝蛋白无毒、无副作用，其粗制品已用于强化营养食品，提纯后应用于肿瘤的激光治疗，可望成为一种有发展前途的光敏剂。

The photodynamic effect of the phycocianin excited with a pulsed copper vapour laser on the human colorectal cancer cell line HR8348 was evaluated in our laboratory. The HR8348 cells were incubatedin RPMI-1640 medium containing a series concentration of phycocianin (100, 50, 25μg/ml), and subsequently subjected to an irradiation with a pulsed copper vapour laser with the wavelength of 620um and energy of 12 J/cm2. 24 hours after irradiation, the survival rates of the cells as determined with MTT assay were 22. 1 %,37. 6% and 89. 7%, respectively, demonstrating a linear doseresponsive effect. Compared with the commercially available photosensitizer chlorophyll Ⅱderivative CPD4, phycocianin appeared to be more effective in killing the HR8348 cells. With its satisfactory nontoxic photosensitivity. phycocyanin would be a refined photosensitizer for the laser therapy for cancer.