摘要
本文应用多聚酶DNA链延伸反应产物限制性酶切片段多态性分析(PCR-RFLP)和单链构象多态性分析(PCR-SSCP)两种方法同时检测了20例胃癌中c-Ha-ras基因突变.本文显示PCR-RFLP和PCR-SSCP均是检c-Ha-ras基因突变的简便、快速和敏感的方法,适用于临床大批量肿瘤标本的检测。
In this work, mutations of c-Ha-ras gene in 20 cases of primary gastric cancer were investigated by using restriction fragments length polymorphism(RFLP) and single-strand conformation polymorphism (SSCP)analysis of the polymerase chain reaction product. We foundthat these two methods were highly simple, rapid and sensitive assay system for detecting c-Ha-ras gene mutations of large number of tumor samples and PCR-SSCP could also detect c-Ha-ras point mutations in several codons at same time.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1995年第1期5-8,共4页
Cancer Research on Prevention and Treatment
基金
"863"基金