胃癌c－Ha－ras基因突变分析方法的建立及临床应用研究

Establishment and Clinical Application Study of C-Ha-ras Gene Mutation AnalysisMethods inGastric Cancer

本文应用多聚酶ＤＮＡ链延伸反应产物限制性酶切片段多态性分析（ＰＣＲ－ＲＦＬＰ）和单链构象多态性分析（ＰＣＲ－ＳＳＣＰ）两种方法同时检测了２０例胃癌中ｃ－Ｈａ－ｒａｓ基因突变．本文显示ＰＣＲ－ＲＦＬＰ和ＰＣＲ－ＳＳＣＰ均是检ｃ－Ｈａ－ｒａｓ基因突变的简便、快速和敏感的方法，适用于临床大批量肿瘤标本的检测。

In this work, mutations of c-Ha-ras gene in 20 cases of primary gastric cancer were investigated by using restriction fragments length polymorphism(RFLP) and single-strand conformation polymorphism (SSCP)analysis of the polymerase chain reaction product. We foundthat these two methods were highly simple, rapid and sensitive assay system for detecting c-Ha-ras gene mutations of large number of tumor samples and PCR-SSCP could also detect c-Ha-ras point mutations in several codons at same time.