5－Fu佐剂包裹物的抗肿瘤作用的实验研究

Experimental Studying on Antitumor Effects of 5-Fu Entrapped by FCA

本文作了福氏完全佐剂（Ｆｒｅｕｎｄ’ｓｃｏｍｐｌｅｔｅａｄｊｕｖａｎｔ，ＦＣＡ）包裹５－Ｆｕ的抗肿瘤作用的实验研究。在对小鼠Ｓ１８０肿瘤（腹水型），ＥＡＣ肿瘤（腹水型）及Ｈ２２肝癌（腹水型）荷瘤鼠的治疗实验中，一次性腹腔注射２ｍｇ～６ｍｇ的５－Ｆｕ（相当于７０公斤人给予７７８～２３３４ｍｇ的剂量）均不能显著延长荷瘤鼠的存活期，盐水ＦＣＡ亦无治疗作用，而５－ＦｕＦＣＡ却显示出显著的治疗效果。４次实验，５－ＦＵ－ＦＣＡ治疗组荷瘤鼠平均存活期３０．６天～４８．３天，均显著长于同次实验的各对照组，且每次实验都有２／８至３／８的荷瘤鼠可完全治愈。

FCA (Freund’s Complete Adjuvant, FCA)is usually used to entrap antigen toimmunize animal,We entrapped 5-Fu with FCA to slow their release in vivo and by means of this to reduce their side effects and to augment their antitumor effects.We called these FCA entrapped 5-Fu, 5-Fu-FCA. The results showed that: the average survival time of S180, EAC and H22 tumor bearing mice treated with 5-Fu was 18.3±1. 6, 17. 9±1. 9 and 19.1±3. 6 days respectively, none of them were cured,while that of 5-Fu-FCA treated groups was 48. 3±21. 1, 30. 6±2. 3 and 33.4±21. 6 days and 3/8,2/8, 2/8 of the tumor bearing mice cured respectively (vs 5-Fu treated groups, P<0. 0001).We conclude that: (1) the adverse effects of 5-Fu could be significantly reduced by means of FCA entrapping; (2)the antitumor effects of 5-Fu could be augmented by means of FCA entrapping.