卡铂对A_（549）肺癌细胞的抗癌作用及其机制的初步探讨

The anticancer effects of carboplatin on A_(549) lung cancer cells and its mechanism

本文观察了卡铂对Ａ_（５４９）肺癌细胞ＤＮＡ合成、ＤＮＡ链间交联的影响及细胞超微结构的改变。依卡铂剂量不同将实验分为Ａ、Ｂ、Ｃ、Ｄ四组，剂量分别为２０μｇ／ｍｌ、４０μｇ／ｍｌ、６０μｇ／ｍｌ、８０μｇ／ｍｌ，另设空白对照Ｅ组。结果表明，卡铂可能是ＤＮＡ模板损伤型药物，通过引起ＤＮＡ交联而阻碍其复制，产生杀细胞效应。

In order to study the anticancer effects of carboplatin (CBDCA) in vitro.We have examined the inhibition of DNA synthesis,DNA interstrand cross-linking(ISC)and the changes of ultrastructure of A549 Lung cancer cells (A549 cells).Four experimental groups, named A (20ug/ml)B(40ug/ ml),C(60ug/ml) and D (80ug/ml)according to different dosage of CBDCA and one group without CBDCA as control were used. In conclusion,we demonstrate that CBDCA is a DNA breakage analog,The DNA ISC induced by CBDCA is related to the inhibition of DNA replication and cytotoxicity.