摘要
本文较系统地作了福氏完全佐剂(Freund'scompleteadjuvant,FCA)包裹5-Fu的抗肿瘤机理的实验研究。FCA包裹5-Fu相的对包封率平均为:84.5%。5-Fu-FCA体外缓释试验结果和Wistar大鼠体内5-Fu及5-Fu-PCA吸收试验均表明:5-Fu用FCA包裹后,在体内外具有缓慢释放的作用。50~200μg浓度的5-Fu在不同作用时间里对肿瘤细胞增殖抑制率随作用时间延长而增加,统计三次实验结果(对H_(22)肝癌细胞),5-Fu200μg/ml作用10分钟对肿瘤细胞增殖抑制率为34.66±6.02;50μg/ml作用24小时的抑制率为61.35±7.17。两者比较差别显著(P<0.08)。结果提示:在肿瘤局部低浓度5-Fu的持续存在,在一定情况下,对肿瘤的抑制作用更强。
FCA (Freund's Complete Adjuvant,FCA)is usually used to entrap antigen to immunize animal,We entrapped 5-Fu with FCA to slow their release in vivo and'by means of this to reduce their side effects and to augment their antituor effects.We called these FCA entrapped 5-Fu, 5- Fu-FCA.The average rate of entrapping 5-Fu by FCA is 84. 5%.The experiment showed that 5- Fu-FCA had a slow release of 5-Fu in vitro and in vivo.The proliferation inhibition rate of H22 tumorcells by higher concentration of 5-Fu (200μg/ml,10min )was 34. 66±6.02,significantly higher than that by lower concentration (50μg/ml)of 5-Fu and the same time(13.46±11.54,P<0.01),but lower than that by lower concentration(50μg/ml) of 5-Fu and longer time(24h,61.35 ±7.18,P<0.008).We conclude that:(1)the release of 5-Fu is markedly reduced in vivo by FCA entrapping;(2)lower concentration of 5-Fu aam longer time may have stronger antitumor effects.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1995年第3期129-132,共4页
Cancer Research on Prevention and Treatment
关键词
氟脲嘧啶
福氏完全佐剂
抗肿瘤
实验研究
Fu (5-Fluorouracilum,5-Fu)
FCA(Freund's Complete Adjuvant,FCA)
MuriueH22 bepato carcinoma
In vitro,In vivo