二亚硝基哌嗪诱发移植于裸鼠的人胚鼻咽粘膜癌变的研究

Carcinogenesis of DNP on Human Embro Nasopharyngeal Mucosa Transplants in Nude 0Mice

人胚鼻咽粘膜移植于裸鼠皮下后可长期存活，并保持其结构和功能，用二亚硝基哌嗪（ＤＮＰ）皮下给药法诱发其癌变。实验分四组，一次性给药组（１１例），低剂量多次给药组（１２例），大剂量多次给药组（１２例），大剂量ＤＮＰ＋促癌剂ＰＭＡ组（１１例）。在４０周内，分别见不典型性增生及异型鳞状化生５、６、６及７例次，原位癌依次为３、１、１及１例，而大剂量ＤＮＰ＋ＰＭＡ组及低剂量多次给药组各诱发浸润癌一例。

Human embro nasopharyngeal mucosa tissues which had been subcutaneously transplanted into nude mice remained their primary structure and function. Based on this experimental model, N'Ndinitrosopiperazine (DNP) was used to induce the transplant mucosa epithelia into cancerous lesion.The experiments had four groups:once DNP administered group (11 case); low dose serial DNP administered group (12 cases); large dose serial DNP administered group (12 case); large dose serial DNP and PEA administered group (11 cases). The result showed that within 40 weeks, there were atypical hyperplasia (5, 6, 5, 4 cases separately); carcinoma in situ (3, 1, 1, 1 cases separately); infiltrating carcinoma (1 case in large dose serial DNP and PMA administered group).