反相高效液相色谱法测定洛美沙星的血和尿药浓度

Determiation of Lomefloxacin In Serum And Urine By Reversed phase HPLC

本文报道用反相高效液相色谱法测定血清和尿液中洛美沙星浓度，固定相用Ｃ＿（１８）柱，流动相为甲醇／０．００８Ｍ磷酸盐缓冲液／０．５Ｍ四丁基溴化铵（２５：７５：４，Ｖ／Ｖ／Ｖ）．荧光检测激发波长２８０ｎｍ，发射波长４５５ｎｍ，以外标法定量。洛美沙星的最低检出限为０．０６μｇ／ｍｌ，线性范围为０．１２５－８．μｇ／ｍｌ．线性关系良好（ｒ＝０．９９９９），日内变异系数２．８７％，日间变异系数２．９４％，回收率７５．８％～８０．９％。

Reversed-phase HPLC was described for the separation and determination of Iomefloxacin inserum and urine. Samples were analysed on an ODS column (7μ, 250×4.6mm) with a mobile phase ofmethand-0.008M phosphate buffer-0.5M tetrabuty lammonium bromide(25: 75 :4,v/v/v).usingfluorescence detection at λ ex 280nm and λ em 455nm.The detection limit was 0. 06μg/ml. GoodIinearity was obtained in the concentration range from 0. 125 to 8μg/ml with the correlation coeffi-cient r= 0. 9999. The average recovery was 75.8%-80.9%and the differences within and betweendays were 2. 87%,2. 94% respectively