湖北光周期敏感核不育水稻农垦58s花药中的某些生化代谢特点

Some Biochemical Metabolic Characters in Nongken 58s Anthers of Hubei Photoperiod Sensitive Genie Male-Sterile Rice

湖北光周期敏感核不育水稻农垦58s单核早期的不育花药的线粒体呼吸速率和LOX活性分别较可育株低11.9％和5％,而单核晚期相应较可育株低18.4％和17.3％。花药发育从单核期至三核期,可育花药的AsA和GSH含量增高,而不育花药仅分别相当于可育株的35—55％和22—32％,并有脂质氢氧化物积累。随着花药发育,可育花药的AsA-POD,GR和6-GPDH活性逐有增高,至三核期达到最高。而不育花药,随花粉败育,在单核早期至三核期,AsA-POD,GR和6-GPDH活性逐渐降低,至三核期,其活性分别为可育株的26％,22％和19％,不育花药的ME和MDH活性亦较可育株低。IDH活性在单核晚期为可育株的47—80％。细胞还原势低是不育花药特征之一。低的还原势可能导致活性氧代谢失调和花药不育。

The respiratary rate in mitochondria and lipoxygenase activity in male-sterile anthers were lower than that of the fertile one by 11.9% and 5% in early uninucleate stage of pollen for HPGMR. respectively. And by 18.4% and 17.3% in late uninucleate stage. As pollen developed from uninucleate to trinucleate stage, AsA and GSH content increased in the fertile anthers. However, that only took 35-55% and 22-32% in male-sterile anthers, respectively, as compared with the fertile one. Lipid hydroperoxide accumlation was also found in male-sterile anthers. AsA-POD. GR and 6-GPDH activities increased gradually as pollen developed from early uninucleate stage to trinucleate stage and maxium activity was found in trinucleate stage. Due to the abortion of pollen in male-sterile anthers. AsA-POD. GR and 6-GPDH activities decreased gradually from early uninucleate to trinucleate stage. In trinucleate stage, these activities only took 26. 19 and 22 percent in male-sterileanthers. respectively, as compared with the fertile one. Decreasing ME and MDH activities were also found in male-sterile anthers. IDH activity decresed and was only 40-80% of that of fertile anthers. The decreasing reduction potential is one of the characterics of male-sterile anthers. And the low reduction potential might cause the uncontroll of activated oxygen and the sterility of anthers.