赖型钩体601株和017株基因文库的构建

CONSTRUCTION OF GENOMIC LIBRARIES OF L. INTERROGANS SEROVAR LAI STRAIN 601 AND STRAIN 017

黄疸出血群赖型钩体601株、017株DNA经EcoRI酶切消化，与EcoRI酶切消化和去磷酸化的pUC18连接后，转化大肠杆菌JM103，建成重组率为85％以上，分别含重组子8700个（601株）和12，000个（017株）的基因文库。随机选择的白色菌落的质位经酶切鉴定表明均含有大小不等的插入DNA片段。

The DNA from L. interrogan serovar lai strain 601 and strain 017 were partially di-gested with restriction endonuclease EcoRI, ligated to plasmid pUC 18 DNA. The DNA from pUC18 had previously been digested with EcoRI and treated with calf alkaline phosphatase. The DNAmixture was transformed into E. coli JM 103. Two genomic libraries were constructed: in the genomiclibrary of strain 601, the recombination frequency was 85%, and there were about 8700 recombina-tion ; in the genomic library of strain 017, the recombination frequency was 86 %, and there were about12000 recombinants. It was confirmed that all selected plasmids of white colonies contained DNA in-serts.