摘要
以河北杨优树无性系一年生枝条为外植体诱导愈伤组织，建立了河北杨体细胞抗盐性突变体离休筛选的实验系统，并以ＮａＣｌ为选择剂，从未经诱变处理及￣（６０）Ｃｏγ－射线或ＥＭＳ诱变处理的愈伤组织材料中，通过一步和多步选择程序进行了抗性愈伤组织的筛选。共得到７个抗性变异系，并均已分化出完整植株。
One-year-old twing of plus tree's clone of Populus hopeiensis was used as the explantto induce callus. The experiment system to selsct salt-resistant mutants of P. hopeiesis was establishedby means of somatic culure in vitro. Using NaCl as selection agent, the mutants have beenseperated through the methods of one-step selection and poly-step selectiom from the callustreated either by ￣(60)Co γ-rays or EMS and the callus with no treatmrnt. 7 resistant variation lineshave been chosen and all of them have regenerated new plants.
出处
《西北林学院学报》
CSCD
1995年第3期1-7,共7页
Journal of Northwest Forestry University
基金
国家"七五"攻关项目
关键词
河北杨
抗盐性
离体筛选
突变体
体细胞
Populus hopeiensis
salt-resistant
experiment system
variation line
selection invitro
