前列腺特异性抗原多充隆抗体的制备及初步应用

THEPRODUCTION AND PRELIMINARYAP-PLICATION OF A POLYCLONAL ANTIBODY TO PROSTATE SPECIFIC ANTIGEN

用盐析法、阴、阳离子交换柱层析及凝胶过滤法，从人前列腺组织中分离纯化前列腺特异性抗原（ＰＳＡ），经ＳＤＳ－ＰＡＧＥ显示其分子最为３４ＫＤ。以该抗原常规兔疫家兔，获得抗ＰＳＡ多克隆抗体，此抗体经进一步纯化后，以免疫印迹技术证实它具有ＰＳＡ＿（３４）特异性。ＡＢＣ法显示该抗体对正常、肥大及癌变的前列腺腺上皮呈阳性反应，而对其它正常及肿瘤组织呈阴性反应。用ＥＬＩＳＡ夹心法分析该抗体血清学特异性，结果表明前列腺癌患者血清ＰＳＡ的含量非常显著地高于对照组。

rostate specific antigen(psA) was purified from the crude tissue extract of fresh humanprostatic tissue by CM-Sephadex-C_(50), Sephadex-C_(100) and DEAE-Sephadex-A_(50) chromatogra-phy, SDS-PAGE showed tnat it had a molecular weight of 34KD Polyclonal antibody(PcAb) was ohained from rabbits immunized with PSA。 The PcAb wasasbsorbed with normal female serum(NFS),precipitated with ammoniuin sulfate and then fol-lowed by DEAE 32 chromatography,The immunoblotting test indicated that it is specific forPSA 34.The immumohistochemical examination showed that the PcAh could stain the prostatictissues(normal, benign hypertrophic and、malignant), but not the other human tissues,Two-Site sandwish enzvme-linked immunosorbent assay(ELISA)indicated that the mean content ofserum PSA in carcinoma of the prostate was significantly higher than that in the normal males.