单纯表达CD_（38）抗原难分类急性白血病细胞起源的研究

Study on Cellular Origin of Unclassified Leukemia Only ExpressedCD_(38) Antigen

采用单克隆抗体免疫酶和聚合酶链反应（ＰＣＲ）技术，研究７例单纯表达ＣＤ＿（３８）抗原难分类急性白血病（ＵＡＬ）化疗前后免疫表型和免疫球蛋白重链（ＩｇＨ）和Ｔ细胞受体（ＴＣＲ）γ基因重排，结果表明：化疗前３例检测到ＴＣＲγ基因重排，化疗后４例表达Ｔ细胞分化抗原，并伴ＴＣＲγ基因重排；１例化疗后表达髓细胞抗原，无ＩｇＨ和ＴＣＲγ基因重排；２例化疗前后仅表达ＣＤ＿（３８）抗原，亦无ＩｇＨ和ＴＣＲγ基因重排。提示动态研究免疫标记有助于ＵＡＬ细胞起源的确定。

Immunophenotype and immunoglobulin heavy chain(IgH)and T cell receptorγ(TCRγ)genesrearrangement in 7 patiens with unclassified acute leukemias whose leukemic cells only expresed CD38 anti-gen were studied by using the immunoenzymatic staining method with monoclonal antibodies and the poly-merase chain reaction(PCR) technique bpfore and after chemotherapy,In 3 patients the leukemic cells weredetected TCRγ gene rearrangement before chemotherapy.After chemotherapy,4 patients expressed T cell dif-ferentiated antigens on their leukemlc cells and all of the 4 patients had TCRγ gene rearrangement.One pa-tient whose leukemic cells expressed myeloid cell antigen and no IgH or TCRγ gene rearrangement.ExpressedCD38 antigen and no IgH or TCRγ gene rearrangement were found only in 2 patients before and afterchemotherapy.The results indicate that dynamic studies on immunologic markers are useful to determiningthe cellular origin of unclassified acute leukemlas.