肝癌及癌旁组织丙肝病毒基因负链研究

Study of the Minus Strand of HCV-RNA in Tumor and Pericancerous Nontumor Liver Tissue in Patients with Hepatocellular Carcinoma

报告运用巢式ＰＣＲ检测５例ＨＣＶ－ＲＮＡ阳性肝癌及癌旁组织和周围血清中ＨＣＶ－ＲＮＡ负链．正链检测：血清及癌旁组织全部阳性，癌组织中３例阳性；负链检测：血清中均阴性，除２例癌组织外癌旁组织５例均阳性。巢式ＰＣＲ是检测ＨＣＶ感染病人外周血中ＨＣＶ－ＲＮＡ非常敏感的一种方法。有助于区别病毒基因是来源于组织本身还是血液中ＨＣＶ粘附于组织上。在肝癌组织中检测到ＨＣＶ－ＲＮＡ负链，推测ＨＣＶ在其中复制，持续感染，有可能构成致癌作用的启动因子，对肝癌的发生起了一定的作用。

Sera and tissue obtained from patients with hepatocellular carcinoma were examined for the presence of hepatitis C virus (HCV) genome by nested polymerase chain reaction (PCR) assay.Whereas plus-stranded HCV-RNA was detectable in sera,hepatocellular carcinoma and pericancerous nontumor liver tissue of type C chronic liver disease Patients,the minus-stranded HCV-RNA was only detectable in the liver tissue.Nested-PCR is a very powerful tool for the detection of HCV-RNA in the sera of patients with HCV infection.It assists in distinguishing between blood viral particles adhering to the tissue and viral RNA contained within the tissue.Detection of the minus-strand of hepatitis C virus RNA by nested-PCR may be implicated in hepatitis C virus replication in infected tissue.This virus may not only function as initiators of hepatocarcinogenesis but may also as a promoter of carcinogenesis.