摘要
应用ＤＭＳＯ（Ｄｉｍｅｔｈｙｌｓｕｌｆｏｘｉｄｅ二甲基亚砜）冷冻割断制样扫描电镜技术首次动态观察了不同感染阶段（ｐｏｓｔＩｎｆｅｃｔｉｏｎ，ＰＩ）实验性单纯疱疹病毒性角膜炎（ＨＳＫ）兔的角膜、三叉神经节（ＴＧ）泪腺等组织的病变情况。初步报告并分析了各组织病变的扫描图像结果，并对冷冻割断法观察扫描电镜的原理及优缺点予以讨论，认为该技术应用于眼部单疱病毒感染的研究图像清晰、直观、简便可行，是一种有实用价值的方法。
his paper reports the appearance of the
corneas, trigeminal ganglia(TG)and lacrimal glands of 20 rab-bits
with primary herpes simplex keratitis as observed by scanning
electron microscopy(SEM)with DMSOfreeze-fracturing technique.At
various post-infection days,the electron micrographs demonstrate for
thefirst time,the tissue response to herpes simplex virus type-1
(HSV-1)infection.We discuss the principle ofthis technique with its
advantages and disadvantages,and conclude that this technique is a
practical one fordetermining HSV-1 effects on ocular tissues in view
of its clear, direct micrographs and simplicity.
出处
《眼科研究》
CSCD
1995年第3期161-163,共3页
Chinese Ophthalmic Research
