摘要
研究了不同膜（ＰＳ，ＰＡＮ）的截流特性及ｐＨ、压强差、浓缩倍数对超氧化物歧化酶（ＳＯＤ）分离纯化的影响．当采用ＰＡＮ膜，浓缩４倍时，ＳＯＤ收率为８５％，比活提高到１３５０Ｕ／ｍｇ。本文还研究了热变性和超滤先后次序对ＳＯＤ纯化的影响。结果表明先超滤、后热变性工艺，ＳＯＤ纯化后总收率６８％，比活达６０７５Ｕ／ｍｇ。
Purification of superoxide dismutase from Pig blood with hollow fiber ultrafiltration was studied in this paper. The membrane type, pH, concentration factor and heat treatment are discussed. When PAN membrane (molecular weight cut off 20000) was used to concentrate the supernatant of the red cell hemolysate for 4 times, thh SOD could be purified 12 times. The recovery and the specific activity of SOD after this process were 68% and 6075U/mg, respectively.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
1995年第9期386-389,共4页
Chinese Journal of Pharmaceuticals
关键词
超氧化物歧化酶
中空纤维超滤膜
分离和纯化
superoxide dismutase, purification, hollow fiber ultrafiltration, separation, Purification
