烧伤后白介素2与白介素2受体基因表达的变化及人参茎叶皂甙的调节作用

Study of the gene expression changes of interleukin 2 and its receptor after burn injury and the modulation effects of ginsenoside in mice

研究了不同程度烧伤小鼠淋巴细胞白介素２（Ｉｎｔｅｒｌｅｕｋｉｎ２，ＩＬ－２）ｍＲＮＡ及ＩＬ－２受体α（ＩＬ－２Ｒα）ｍＲＮＡ的变化，并观察人参茎叶皂甙的体内外调节作用。结果显示，烧伤后ＩＬ－２ｍＲＮＡ及ＩＬ－２ＲαｍＲＮＡ水平均降低，且同伤情变化关系密切。伤后ＩＬ－２及ＩＬ－２Ｒα的变化分别同ＩＬ－２ｍＲＮＡ、ＩＬ－２ＲαｍＲＮＡ的变化呈非常显著的正相关。人参茎叶皂甙皮下注射（５０ｍｇ·ｋｇ（－１）·ｄ（－１），连续４ｄ）可显著提高烧伤小鼠淋巴细胞ＩＬ－２ｍＲＮＡ及ＩＬ－２ＲαｍＲＮＡ水平，并促进ＩＬ－２生成及ＩＬ－２Ｒα表达。人参茎叶皂甙体外（０．１至１００μｇ／ｍｌ）也可拮抗烧伤小鼠ＩＬ－２ｍＲＮＡ及ＩＬ－２ＲαｍＲＮＡ的受抑状态。上述结果表明，烧伤可致淋巴细胞ＩＬ－２及ＩＬ－２Ｒα的基因表达受抑，进而导致ＩＬ－２的生成及ＩＬ－２Ｒα的表达减少。人参茎叶皂甙对这一变化具有明显的拮抗效应。

The changes of interleukin 2(IL-2 ) mRNA and its receptor α(IL-2Rα) mRNA of lymphocytes were studied in mice with different degrees of burn injury and the modulation effects of ginsenoside from the ginsen stem and leaves were observed in vivo and invitro.It was found that the levels of IL-2mRNA and IL-2Rα mRNA were reduced after burn injury and the reduction was related to the severity of burn injury.There was a significant correlation between the levels of IL-2 and IL-2 mRNA and those of IL-2Rαand IL-2Rα mRNA postburn, Subcutaneous administration of ginsenoside of the dosage 50 mg·kg-1·d-1 for 4 consecutive days after burns could significantly increase the levels of IL-2mRNA and IL-2Rα mRNA and enhance the production of IL-2 and the expression of IL-2Rα.Ginsenoside of the dosage 0.1～100 μg/ml could alleviate the suppression of IL-2 mRNA and IL-2Rα mRNA after burn injury in vitro.These findings suggest that burn injury can suppress the gene expression of IL-2 and IL-2Rα of lymphocytes and ginsenoside exerts excellent therapeutic effects on these changes.