粉防已碱对大鼠腹腔巨噬细胞释放部分炎症介质的影响

Study of tetrandrine effects on several inflammation mediators released by peritoneal macrophages of rats

血小板活化因子（ＰＡＦ）、肿瘤坏死因子（ＴＮＦ）及白介素－１（ＩＬ－１）是几个很强的炎症介质，对多种细胞、组织及器官都有重要的作用。利用洗涤的兔血小板聚集的方法和酶联免疫法、小鼠胸腺细胞增殖法分别测定了粉防已碱（Ｔｅｔ）对脂多糖（ＬＰＳ）刺激大鼠腹腔巨噬细胞（ＰＭΦ）释放ＰＡＦ、ＴＮＦ及ＩＬ－１的影响，结果表明Ｔｅｔ对ＴＮＦ、ＩＬ－１及ＰＡＦ的释放都有显著地抑制作用，并在０．１～１００μｍｏｌ／Ｌ有剂量依赖关系，这提示Ｔｅｔ的抗炎、免疫抑制作用的机理之一是由于Ｔｅｔ抑制了ＰＡＦ、ＩＬ－１、ＴＮＦ等炎症介质的释放。

Platelet-activating factor (PAF),tumor necrosis factor(TNF)and interleukin 1(IL-1)are potent molecular mediators of imflammation and exert significant effects on various types of cells,tissues and organs.The effects of tetrandrine(Tet)on the PAF,TNF and IL-1 released by the peritoneal macrophages of rats were observed with the methods of washed rabbit platelets,mouse thymocyte proliferation and enzyme-linked immunosorbant assay respectively.It was found that the release of PAF,TNF and IL-1 were significantly inhibited by Tet in a dosage-dependent manner of the range 0.1～100 μmol/L,whch indicates that one of the mechanisms of the anti-inflammatory effect of Tet is its inhibition to the release of PAF,TNF and IL-1 by the peritoneal macrophages.