血清脯肽酶与肝损害

The relationship of serum prolidase and liver damage in mice

采用ＣＣｌ４致小鼠急、慢性肝损伤模型，平行动态观察了肝组织学改变与同期血清脯肽酶（ＰＬＤ）和丙氨酸转氨酶（ＡＬＴ）变化关系。急性肝损伤中，血清ＰＬＤ和ＡＬＴ活性均明显升高，且二者变化呈显著正相关（ｎ＝１００，ｒ＝０．３８５３．Ｐ＜０．０１）。ＰＬＤ峰值期为注射ＣＣｌ４后１６ｈ，与肝坏死最严重的时相一致。ＰＬＤ峰值和恢复时间均早于ＡＬＴ。肝硬化形成大致可分为胶原纤维增生（１～６周），肝硬化形成（６～９周）和肝硬化（９～１２周）３个阶段。其间血清ＰＬＤ活性第１周开始升高、３～６周达高峰，而后开始下降，９周后降至正常，而ＡＬＴ无明显变化。结果表明，血清ＰＬＤ活性测定是一项诊断急性肝损伤的敏感指标，除外急性肝损伤、ＰＬＤ活性在某种程度能较好地反映进展性肝纤维化。

The activity of serum prolidase(PLD)and alanine aminotransferase(ALT)was determined and the histological changes of the liver was observed on a murine model of hepatocyte damage and fibrosis induced with CCl4,In the acute hepatocyte damage,the activity of PLD and ALT was significantly increased with a positive correlation between the 2 enzymes(n=100,r=0.3853,P<0.01).The peak elevation of ALD was in the 16th h, corresponding to the time of most severe lysis of hepatocytes.The time of peak elevation and recovery of PLD was earlier than that of ALT. The chronic hepatocyte damage and fibrosis could roughly be divided into fibroproliferating stage(1st to 6th week),cirrhosis developing stage(6th to 9th week)and cirrhosis stage(9th to 12th week). There was no significant correlation between the changes of PLD and ALT(n=88,r=0.132,P>0.05)and no significant changes of ALT was observed.The PLD activity began to elevate in the lst week,reached the peak in the 4th week and returned to the normal range after 9th week.These findings suggest that serum activity of both PLD and ALT is a goog biochemical index to diagnose acute hepatocyte injury.When the acute phase progresses into the chronic phase,the activity of PLD only can reveal the development of cirrhosis.