地高辛标记探针原位杂交对肝细胞癌HBV　DNA分布的研究

Identification of hepatitis B Virus-DNA in human hepatocellular carcinoma with in situ hybridization using a degoxigenin-labelled probe

为了探讨ＨＢＶ与肝细胞癌（ＨＣＣ）发病的关系，作者应用地高辛标记ＨＢＶＤＮＡ探针原位杂交及免疫组化检测了８０例ＨＣＣ组织及癌周肝组织的ＨＢＶＤＮＡ、ＨＢｓＡｇ。结果显示，癌周肝组织ＨＢＶ阳性６３例（７９％），癌组织ＨＢＶ阳性４９例（６０％），癌周阳性细胞数明显多于癌内。癌周ＨＢＶＤＮＡ，以复制型为主，而癌组织的ＨＢＶＤＮＡ大多属整合型。结果表明大多数ＨＣＣ病例与ＨＢＶ存在密切相关，而且整合型ＨＢＶＤＮＡ在ＨＣＣ发生过程中有着重要的作用。

In order to study the relationship of hepatitis B virus(HBV)with hepatocellular carcinoma(HCC),HBV and HBsAg were detected in the sections of paraffin-embedded tissue specimens of 80 cases of HCC with in situ hybridization and immunohistochemical assay.It was found that the positive rate of HBV DNA was 79%(63/80)in the peri-carcinoma liver tissue and 60%(49/80) in the cancerous tissue.The number of HBV DNA positive cells was significantly larger in the peri-carcinoma tissue than in the cancerous tissue.HBV DNA might be of the replicative form in the peri-carcinoma tissue but of the integrated form in the cancerous tissue.These findings suggest that the pathogenesis of HCC is closely related to HBV infection and HBV integration might play an important role in hepatocarcinogenesis.