抗丙型肝炎病毒核心抗原单克隆抗体杂交瘤细胞株的建立及初步鉴定

Establishment and preliminary identification of a hybridoma cell line producing monoclonal antibodies against core antigen of hepatitis C virus

用丙型肝炎病毒（ＨＣＶ）核心区合成肽作抗原免疫ＢＡＬＢ／ｃ小鼠，取其脾细节与骨髓瘤细胞ＳＰ＾２／０进行融合。经有限稀释法克隆３代后获得一株抗ＨＣＶ核心抗原ＣＰ１０的单克隆抗体（ＭｃＡｂ）杂交瘤细胞株３Ｅ７，并对其分泌抗体进行初步鉴定。杂交瘤细胞培养上清的抗体滴度为１：３２０，诱生同系小鼠腹水的滴度为１：１２８００，该ＭｃＡｂ能与ＣＰ１０特异性结合，杂交瘤细胞染色体数目为１０６条。

A hybridoma cell line to produce monoclonal antibodies against the core antigen of hepatitis C virus was established by the fusion of SP￣2/0 mouse myeloma cells with the splenocytes of BALB/c mouse immunized with the synthetic peptide of the core region of hepatitis C virus(HCV).The titer of the culture supernatant was 1∶320 and that of the ascites from the mouse with transplanted hybridoma was 1: 12800.The specificity and afflnity of the monoclonal antibodies were preliminarily identified with neutralization inhibition test and ELISA. It was shown that the monoclonal antibodies was IgG in nature and the hybridoma was consisted of 106 chromosomes.