实验性大鼠急性心肌缺血的酶组织化学观察

HISTOCHEMICAL OBSERVATION ON EXPERIMENTAL RAT'S EARL MYOCARDIAL INFARCTION

本文报告50只雄性Wistar大鼠结扎左冠状动脉所致早期急性心肌梗死（缺血1～8h的组织化学变化。结果发现：糖原含量在缺血1h、NADHD和LDH活性在缺血2h梗死区明显下降；缺血4h，酶活性下降发展到全部缺血区。CCo.CK及ATP酶活性下降在缺血4h明显，缺8h发展到整个缺血区。非缺血区NADHD、LDH、CK、ATP酶活性能耐受死后自溶的影响达24h以上，具有法医学实践意义。糖元（PAS染色）和PPr在死后早期降解快，非缺血区与缺血区无显著性差异，因此缺乏法医病理学应用意义。

This Paper reports the histochemical changes occcured at the early acute cardial ischemic area in-duced experimentally by ligation of left coronary artery of 50 male Wistar rats. The following histo-chemical changes at the ischemical areas were observed: obvious decrease of glycogen of cardical is-chemical for 1h, of both NADHD and LDH activity of ischemical for 2h as well as CCo,CK and ATP activity of ischemical for 4h. The decrease of CCo,CK and ATP activity propagated to the whole is-chemical area at the time of ischemical for 8h. The activity of NADHD,LDH'CK and ATP can toler-ated the postmortem autolysis influence up to 24h after death.