抗人心肌肌凝蛋白重链单克隆抗体在心肌缺血尸检例中的应用

EXAMINATION OF MYOCARDIAL ISCHEMIA IN AUTOPSY CASES BY IMMUNOHISTOCHEMICAL METHOD WITH MONOCLONAL ANTIBODY AGAINST HUMAN MYOCARDIAL MYOSIN-H CHAIN

为了更有效地检测早期及超早期心肌缺血性病变，对5例心肌组织材料进行了肌凝蛋白重链（MyosinH链）及肌红蛋白的免疫组织学染色，探讨了肌凝蛋白的染色情况、抗原分布、死后变化对染色的影响及心肌早期、超早期缺血的有无、程度及与死亡的关系。应用石蜡组织切片进行的抗人心肌肌凝蛋白重链免疫组织学染色，在病理学方面尚属首次，这种单克隆抗体对人的特异性高，无共集现象，在心肌缺血数分钟乃至十几分钟后即可发现缺血心肌细胞中肌凝蛋白重键的脱失像，是分析早期、超早期心肌缺血所致急死及其它原因死亡案例心肌缺血病变的有力方法。

Examination of myocardial ischemia in 5 autopsy cases was carried 0ut by immunohistochemicalmethod with monoclonal antibody raised against the human myocardial myosin-H chain and myo-globln. Loss of myocardial myosin-H chain was found several or more than two minutes after coronaryartery ligation. The condition of the immuno-staining methed, localization of the myosin antigen, in-fulence of the postmortem change on the immuno-staining method as well as the correlation of the ear-ly very early myocardial ischemia and the sudden unexpectcd death were discussed. The monoclonalantibedy was specific. No false positive immuno-staining was found. This is an effective methed to di-agnose the early and very early myocardial ischemia leading to sudden unexpected death. This methedis firstly reported.