小白鼠肝脏5’—核苷酸酶活力与死亡时间的关系

A STUDY ON THE CORRELATION BETWEEN THE 5'-NUCLEOTIDASE ACTIVITY IN MOUSE LIVER AND THE TIME ELAPSED AFTER DEATH

小白鼠死亡后，肝脏不离体恒温保存，定时定量制备肝浸出液为酶样品，催化5’-腺苷酸（5’AMP）生成腺苷和无机磷酸，后者在还原利存在下与钼酸试剂发生蓝色反应，其颜色深度与磷酸含量成正比。用722型分光光度计测定光密度（OD值），计算酶活力。在三种温度下，经5次重复实验，发现小白鼠死亡后在3O℃12小时、18℃21小时、7℃24小时，其酶活力降低均呈明显的规律性。说明此酶用于一定温度和时间范围内的死亡时间推断具有参考价值。

The constant volume of liver extract was made from the dead mice kept in the comstant tempera-true as a sample of 5' -nucle0tidase. It catalyzes 5' -AMP preducing adnosine and inorganic phosphoricacid. The later reacted with molybdic acid reagent developing blue coorl- The intensity of blue color isproportional to the quantity of the phosphoric acid. The activity of 5' -nucleotidase is calculated by theoptical density of 722 type spectrophotometer, ln the present study which was repeated for fiveVtimes, the regular decline of the activity of 5' -nucleotidase was observed at 12h (3O°C ), 21h (18 °C )and 24h(7°C ) respectively after death. This study indicated that this methed has a value in estimatingthe time of death in a range of the certain time and temperatrue.